Sequencing Datasets

Download and analyze sequencing datasets for greater insight into a range of DNA and RNA sequencing (RNA-Seq) applications. Bases2Fastq Software generated all FASTQ files from Element AVITI™ System bases files.

2 x 300 Sequencing

Application	Chemistry	Dataset	Key Resource
16S amplicon	Cloudbreak Freestyle™	ZymoBIOMICs Microbial Community Standard	Not applicable
16S amplicon	Cloudbreak™	ZymoBIOMICs Microbial Community Standard	Not applicable
B-cell receptor (BCR)	Cloudbreak	Spleen and PMBC	Not applicable
Human WGS	Cloudbreak	Human reference genome with the Adept™ Workflow	Whole-Genome Sequencing web page
Human WGS	Cloudbreak	Human reference genome with the Elevate™ Workflow	Whole-Genome Sequencing web page

RNA Sequencing

Application	Chemistry	Dataset	Key Resource
RNA-Seq, Elevate	Cloudbreak Freestyle	Universal Human Reference RNA
RNA-Seq, third-party	Cloudbreak Freestyle	Universal Human Reference RNA
Bulk RNA-Seq	Cloudbreak	Universal Human Reference RNA with an ERCC spike-in	Not applicable
MicroRNA (miRNA) sequencing	Cloudbreak	Human reference RNA	AVITI and miRNA application note
Whole-transcriptome gene expression	Cloudbreak	10x Genomics Chromium Single Cell Gene Expression Flex (human PBMC cells)	Single-cell sequencing and AVITI blog post

Single-Cell Sequencing

Application	Chemistry	Dataset	Key Resource
Single-cell gene expression	Cloudbreak Freestyle	10x Genomics Chromium Single Cell Gene Expression Flex (PBMC, human T-cells, BBMC)	AVITI and 10x Genomics single-cell application note
Single-cell gene expression	Cloudbreak	10x Genomics Chromium Single Cell Gene Expression Flex	AVITI and 10x Genomics single-cell application note
Single-cell 3’ gene expression	Version 1	10x Genomics Chromium Single Cell 3’	AVITI and 10x Genomics single-cell application note

Whole-Exome Sequencing

Application	Chemistry	Dataset	Key Resource
Human WES	Cloudbreak Freestyle	Twist Bioscience Exome 2.0 (NA12878)	Cost-effective sequencing to understand genetic disease blog post
Human WES	Cloudbreak	Twist Bioscience Exome 2.0 (NA12878)	Cost-effective sequencing to understand genetic disease blog post

Whole-Genome Sequencing

Application	Chemistry	Dataset	Key Resource
Bacterial WGS	Cloudbreak	Microbial genome reference set	Human and microbial genomes with KAPA & AVITI application note
Human WGS	Cloudbreak Freestyle	Human reference genomes	Accurate human genome analysis with Avidity Sequencing ™ preprint (results)
Human WGS	Cloudbreak	Human reference genomes	Accurate human genome analysis with Avidity Sequencing preprint (results)
Human WGS	Version 1	Human reference genome	Not applicable
Human methylation WGS	Cloudbreak	NEBNext Enzymatic Methyl-seq (NA12878)	Less expensive methylation detection blog post
Human methylation WGS	Version 1	NEBNext Enzymatic Methyl-seq (NA12878)	Less expensive methylation detection blog post (results)