Sequencing Datasets

Download and analyze sequencing datasets for greater insight into a range of DNA and RNA sequencing (RNA-Seq) applications. Bases2Fastq Software generated all FASTQ files from Element AVITI™ System bases files.

2 x 300 Sequencing

Application	Chemistry	Dataset	Key Resource
16S amplicon	Cloudbreak Freestyle™	ZymoBIOMICs Microbial Community Standard	Improved quality for 16S and metagenome sequencing (poster)
16S amplicon	Cloudbreak™	ZymoBIOMICs Microbial Community Standard	Improved quality for 16S and metagenome sequencing (poster)
B-cell receptor (BCR)	Cloudbreak	Spleen and PMBC	Not applicable
Human WGS	Cloudbreak	Human reference genome with the Adept™ Workflow	Whole-Genome Sequencing web page
Human WGS	Cloudbreak	Human reference genome with the Elevate™ Workflow	Whole-Genome Sequencing web page

RNA Sequencing

Application	Chemistry	Dataset	Key Resource
RNA-Seq, third-party	Cloudbreak Freestyle	Universal Human Reference RNA
Bulk RNA-Seq	Cloudbreak	Universal Human Reference RNA with an ERCC spike-in	Not applicable
MicroRNA (miRNA) sequencing	Cloudbreak	Human reference RNA	AVITI and miRNA application note
Whole-transcriptome gene expression	Cloudbreak	10x Genomics Chromium Single Cell Gene Expression Flex (human PBMC cells)	Single-cell sequencing and AVITI blog post

Single-Cell Sequencing

Application	Chemistry	Dataset	Key Resource
Single-cell gene expression	Cloudbreak Freestyle	10x Genomics Chromium Single Cell Gene Expression Flex (PBMC, human T-cells, BBMC)	AVITI and 10x Genomics single-cell application note
Single-cell gene expression	Cloudbreak	10x Genomics Chromium Single Cell Gene Expression Flex	AVITI and 10x Genomics single-cell application note
Single-cell 3’ gene expression	Version 1	10x Genomics Chromium Single Cell 3’	AVITI and 10x Genomics single-cell application note

Whole-Exome Sequencing

Application	Chemistry	Dataset	Key Resource
Human WES	Trinity™	IDT xGen™ Hyb Capture Kit Trinity	Infographic: Trinity Targeted Sequencing Workflow
Human WES	Trinity	Twist for Element Trinity, Exome 2.0 + Comp Spike, Fast Hyb Workflow	Infographic: Trinity Targeted Sequencing Workflow
Human WES	Trinity	Twist for Element Trinity, Exome 2.0 + Comp Spike, Standard Hyb Workflow	Infographic: Trinity Targeted Sequencing Workflow
Human WES	Cloudbreak Freestyle	Twist Bioscience Exome 2.0 (NA12878)	Cost-effective sequencing to understand genetic disease blog post
Human WES	Cloudbreak	Twist Bioscience Exome 2.0 (NA12878)	Cost-effective sequencing to understand genetic disease blog post

Whole-Genome Sequencing

Application	Chemistry	Dataset	Key Resource
Human WGS	Cloudbreak Freestyle	Human reference genomes	Accurate human genome analysis with Avidity Sequencing ™ preprint (results)
Bacterial WGS	Cloudbreak UltraQ™	E. coli whole-genome	Cloudbreak UltraQ blog
Human WGS	Cloudbreak UltraQ	Human cell line (CHM13)	Cloudbreak UltraQ blog
Human WGS	Cloudbreak	Human reference genomes	Accurate human genome analysis with Avidity Sequencing preprint (results)
Human WGS	Version 1	Human reference genome	Not applicable
Human methylation WGS	Cloudbreak	NEBNext Enzymatic Methyl-seq (NA12878)	Less expensive methylation detection blog post
Human methylation WGS	Version 1	NEBNext Enzymatic Methyl-seq (NA12878)	Less expensive methylation detection blog post (results)

Metagenome Sequencing

Application	Chemistry	Dataset	Key Resource
Metagenome Mock Community	Cloudbreak	Mock community metagenome sequencing	Improved quality for 16S and metagenome sequencing (poster)
16S amplicon	Cloudbreak Freestyle™	ZymoBIOMICs Microbial Community Standard	Not applicable
16S amplicon	Cloudbreak™	ZymoBIOMICs Microbial Community Standard	Not applicable
16S amplicon	Cloudbreak, Cloudbreak Freestyle	16S V3-V4 Amplicon 2x300	Improved quality for 16S and metagenome sequencing (poster)
Shotgun metagenome	Cloudbreak	Shotgun Metagenome 2x150	Improved quality for 16S and metagenome sequencing (poster)