Single-Cell Gene Expression

Cloudbreak™ chemistry performed single-cell gene expression sequencing, profiling fixed samples. 


The 10x Genomics Chromium Single Cell Gene Expression Flex prepared libraries from human cells. The Adept™ Library Compatibility Kit v1.1 circularized the libraries, which were then pooled to target ~10,000 reads per cell. The AVITI™ System performed a 28 x 90-cycle run and Bases2Fastq generated the FASTQ files. 10x Genomics prepared the samples and libraries, which Element sequenced.


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