16S Amplicon Sequencing, Third Party (Cloudbreak Freestyle™)

Cloudbreak Freestyle chemistry was used to perform amplicon sequencing of the 16S ribosomal (rRNA) gene.

Methods

The V3 and V4 regions of bacterial 16S rRNA genes were PCR-amplified from a ZymoBIOMICS Microbial Community DNA Standard using KAPA HiFi HotStart ReadyMix. IDT xGen indexing primers were added using amplification. Libraries were pooled as a 64-plex and sequenced on the AVITI™ System using Cloudbreak Freestyle chemistry with a 2 x 300 read length. Bases2Fastq was used to generate FASTQ files.

Samples

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