Human Whole-Genome Sequencing, Third Party (Cloudbreak Freestyle™)

Cloudbreak Freestyle chemistry was used to perform whole-genome sequencing (WGS) of human reference genomes. 


Four total PCR-free libraries were prepared from input of 1 µg of HG001 and HG002 gDNA using the KAPA HyperPlus Kit. The samples were mechanically sheared to two different insert sizes (~400 bp and ~500 bp), end-repaired, and ligated with KAPA unique dual index (UDI) adapters. Libraries were sequenced using Cloudbreak Freestyle chemistry on the AVITI™ System with a 2 x 150 read length. Bases2Fastq was used to generate the FASTQ files. 


Enter your email to access datasets for each sample.

By submitting this form, you agree to our Privacy Policy and Terms and Conditions.