Single-Cell Gene Expression, Third Party (Cloudbreak Freestyle™)

Cloudbreak Freestyle chemistry was used to perform single-cell gene expression sequencing, profiling fixed samples. 


The 10x Genomics Chromium Single Cell Gene Expression Flex was used to prepare libraries from human peripheral blood mononuclear cells (PBMC), human T cells, and baboon bone marrow cells (BBMC). Libraries were pooled to target ~10,000 reads per cell and sequenced using Cloudbreak Freestyle chemistry on the AVITI™ System using a 28 x 90-cycle run. Bases2Fastq was used to generate FASTQ files. 10x Genomics prepared the samples and libraries, which Element sequenced. 


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