Human Methylation Whole-Genome Sequencing (Cloudbreak™)

Cloudbreak chemistry performed human methylation whole-genome sequencing (WGS) of reference genomic DNA (gDNA), preserving the methylation status of cytosine residues.


NA12878 was mixed with methylated pUC19 and unmethylated Lambda phage DNA, then sheared to a fragment size of ~250 bp. Two replicates of 200 ng sheared DNA were input for the NEBNext Enzymatic Methyl-seq Kit, which was paired with the NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs). Samples were amplified, circularized with the Adept™ Library Compatibility Kit v1.1, and pooled equally. The AVITI™ System performed a 2 x 150 run and Bases2Fastq generated the FASTQ files.


Enter your email to access datasets for each sample.

By submitting this form, you agree to our Privacy Policy and Terms and Conditions.