Human Whole-Exome Sequencing, xGen™ Hyb Capture Kit for Element, 2 hr Fast Hyb Workflow (Trinity™ Sequencing Kit)

The Trinity sequencing kit was used to perform whole-exome sequencing (WES) on reference genomic DNA (gDNA).

Methods

The xGen Exome Sequencing Kit Trinity for Element (#10022463) was used to prepare libraries and perform capture for Trinity sequencing according to the user guide with some protocol modifications.

Libraries were prepared from 100 ng of NA12878 gDNA, with 7 PCR cycles performed for amplification and indexing of the replicates. An additional 5 cycles of end-polishing PCR were performed on these libraries individually. A total of 24 libraries were pooled equally by mass (416.67 ng per sample) in a single 2 hr hybridization capture reaction.

Following capture, the libraries were sequenced using the Trinity 2x75 Sequencing Kit (#860-00019). Bases2Fastq was used to generate FASTQ files from the sequencing data.

Samples

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