Application Note

SC RNA-Seq with 10x Genomics & Element AVITI™ System

Screenshot of application note for Element AVITNew technologies that enable scientists to genetically profile thousands of cells individually using next-generation sequencing (NGS) have transformed our understanding of developmental biology and disease pathology. The 10x Chromium Single Cell 3’ assay (Chromium Single Cell Gene Expression) enables such investigations at scale. Because the assay uses NGS as readout, novel sequencing solutions that offer high quality, low cost, and flexibility of design are needed.

Element's AVITI™ System provides such a solution, with performance features that make NGS more accessible to labs than ever before. At its core the AVITI System uses a novel Avidite chemistry that enables a combination of exceptional accuracy, low cost, and operational efficiency. The instrument features two independent flow cells designed to work seamlessly in any NGS workflow.

This application note describes a study that pairs the 10x Genomics Chromium Single Cell 3’ assay with the Element AVITI System to provide a comprehensive solution for single cell sequencing. Two libraries created from human peripheral blood mononuclear cells (PBMCs), which query different cell counts, are used for the demonstration.

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