16S Amplicon Sequencing, Third Party (Cloudbreak Freestyle™)

Cloudbreak Freestyle chemistry was used to perform amplicon sequencing of the 16S ribosomal (rRNA) gene. 


The V3 and V4 regions of bacterial 16S rRNA genes were PCR-amplified from a ZymoBIOMICS Microbial Community DNA Standard using KAPA HiFi HotStart ReadyMix. IDT xGen indexing primers were added using amplification. Libraries were pooled as a 64-plex and sequenced on the AVITI™ System using Cloudbreak Freestyle chemistry with a 2 x 300 read length. Bases2Fastq was used to generate FASTQ files. 


Enter your email to access datasets for each sample.

By submitting this form, you agree to our Privacy Policy and Terms and Conditions.