16S Amplicon Sequencing

Cloudbreak chemistry performed amplicon sequencing of the 16S ribosomal (rRNA) gene.


The V3 and V4 regions of bacterial 16S rRNA genes were PCR-amplified from a ZymoBIOMICS Microbial Community DNA Standard using KAPA HiFi HotStart ReadyMix. Adept libraries were pooled as a 64-plex and sequenced on the AVITI™ System using a 2 x 300 read length. Bases2Fastq generated the FASTQ files.


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